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Fig. 5b, Fig. 5c, Electron micrographs of pellet cultures prepared using human monolayer-expanded chondrocytes. The pellets were cultured for two days in DMEM/ITS+/TGF-b1 and subsequently maintained for 12 days in a) DMEM/10%FCS (x6000) or b) DMEM/ITS+/TGF-b1 (x6000). Figure 5c is taken from the bottom right hand corner of Figure 5b (x26000). This indicates the presence of collagen fibrils in the extracellular matrix (arrow).
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Autologous chondrocyte implantation